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Literature summary extracted from

  • Chen, M.; Kato, K.; Kubo, Y.; Tanaka, Y.; Liu, Y.; Long, F.; Whitman, W.B.; Lill, P.; Gatsogiannis, C.; Raunser, S.; Shimizu, N.; Shinoda, A.; Nakamura, A.; Tanaka, I.; Yao, M.
    Structural basis for tRNA-dependent cysteine biosynthesis (2017), Nat. Commun., 8, 1521 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.27 expressed in Escherichia coli B834(DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.73 structure of the SepCysE-SepCysS-tRNACys ternary complex, at a resolution of 2.6 A by molecular replacement. SepCysS recognizes the discriminator base U73 of tRNACys Methanocaldococcus jannaschii
6.1.1.27 hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.0, 0.3M ammonium sulfate, 36% (w/v) 2-methyl-2,4-pentanediol Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.73 Methanocaldococcus jannaschii Q59072
-
-
2.5.1.73 Methanocaldococcus jannaschii DSM 2661 Q59072
-
-
6.1.1.27 Methanocaldococcus jannaschii Q59054
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys
-
 Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.27 homotetramer
-
 Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
6.1.1.27 O-phosphoseryl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.27 SepRS
-
 Methanocaldococcus jannaschii
6.1.1.27 sepS
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.27 ATP
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.5.1.73 physiological function the three domains of enhancer SepCysE each bind O-phosphoseryl-tRNA synthetase SepRS, Sep-tRNA:Cys-tRNA synthase SepCysS, and tRNACys, respectively, which mediates the dynamic architecture of the transsulfursome and enables a global long-range channeling of tRNACys between SepRS and SepCysS distant active sites Methanocaldococcus jannaschii